Genetic Association of PPARGC1A Gene Single Nucleotide Polymorphism with Milk Production Traits in Italian Mediterranean Buffalo.

Seyed Mahdi Hosseini,Bianca Gasparrini,Angela Salzano,Farmanullah Farmanullah,Majid Pasandideh,Giuseppe Campanile,Aixin Liang,Guohua Hua,Ye Tingzhu,Sayed Haidar Abbas Raza,Nicola M Schreurs,Liguo Yang

doi:10.1155/2021/3653157

Abstract

PPARGC1A gene plays an important role in the activation of various important hormone receptors and transcriptional factors involved in the regulation of adaptive thermogenesis, gluconeogenesis, fiber-type switching in skeletal muscle, mitochondrial biogenesis, and adipogenesis, regulating the reproduction and proposed as a candidate gene for milk-related traits in cattle. This study identified polymorphisms in the PPARGC1A gene in Italian Mediterranean buffaloes and their associations to milk production and quality traits (lactation length, peak milk yield, fat and protein yield, and percentage). As a result, a total of seven SNPs (g.-78A>G, g.224651G>C, g.286986G>A, g.304050G>A, g.325647G>A, g.325817T>C, and g.325997G>A) were identified by DNA pooled sequencing. Analysis of productivity traits within the genotyped animals revealed that the g.286986G>A located at intron 4 was associated with milk production traits, but the g.325817T>C had no association with milk production. Polymorphisms in g.-78A>G was associated with peak milk yield and milk yield, while g.304050G>A and g.325997 G>A were associated with both milk yield and protein percentage. Our findings suggest that polymorphisms in the buffalo PPARGC1A gene are associated with milk production traits and can be used as a candidate gene for milk traits and marker-assisted selection in the buffalo breeding program.

Highlights

Water buffalo provides 5% of the world’s milk supply, and their milk is high in protein, fat, lactose, and minerals compared to cow’s milk
PPARGC1A gene plays an important role in the activation of various important hormone receptors and transcriptional factors involved in the regulation of adaptive thermogenesis, fiber-type switching in skeletal muscle, mitochondrial biogenesis, adipogenesis, and gluconeogenesis [17, 22, 23]
A total of 7 single nucleotide polymorphisms (SNP) were detected in the PPARGC1A gene of which one was located in the promotor, 4 in exons, and 2 in introns of chromosome 7 in Italian Mediterranean buffalo (Table 1)

Summary

Introduction

Water buffalo provides 5% of the world’s milk supply, and their milk is high in protein, fat, lactose, and minerals compared to cow’s milk. There are two types of domestic water buffalo, the riverine buffalo (Bubalus bubalis, 2n = 50) and the swamp buffalo They differ significantly in milk yield, approximately 2200-2400 kg/year for the riverine buffalo and 500-700 kg/year for the swamp buffalo [3]. European buffalo is all of the river types, and in Italy, the Mediterranean type is selected from other European breeds but differs genetically [4]. Mediterranean buffalo in Italy produces more than

Methods

Results

Conclusion