Abstract
SUMMARY The genetic and antigenic nature of feline cell-associated herpesvirus (Fecahv) was characterized by use of dna restriction endonuclease analysis, and direct and indirect fluorescent antibody (fa) techniques. Serologic responses of 6 conventionally reared cats with induced Fecahv urinary tract infection were retrospectively evaluated, using an indirect fa test. The EcoRI, HindIII, and Pst I restriction endonuclease cleavage patterns of Fecahv dna were similar to those of bovid herpesvirus 4 (bhv-4; DN599 strain) dna. Specific fluorescence was observed when Fecahv-inoculated cell monolayers were reacted with fluorescein-conjugated bhv-4 (DN599 strain) antiserum. Conversely, specific fluorescence was also observed when feline anti-Fecahv serum and fluorescein-conjugated caprine anti-feline IgG was reacted with bhv-4 (DN599 strain)-infected cell monolayers. At postinoculation week 10, serum antibody titer in cats with Fecahv-induced urinary tract infection ranged from 1:2,560 to 1:10,240, as measured by use of indirect fa testing. It was concluded that Fecahv is a member of the bhv-4 group. In addition, the Fecahv indirect fa test provides a sensitive and specific means of evaluating Fecahv antibody concentration in exposed cats.
Published Version
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