Abstract
Introduction: Escherichia coli is one of the most prevalent bacterial species which cause gastrointestinal and digestive tract infections in humans and livestock. This study examined genotypic diversity of the E. coli isolates taken from fecal specimens in Zabol using random amplification of polymorphic DNA (RAPD) method and phylogenetic background.Materials and Methods: In this study, 100 isolates were collected from human samples and identified by the common biochemical tests. The isolates were categorized into four main phylogenetic groups including group A (28 isolates), group B1 (7 isolates), group B2 (48 isolates), and group D (17 isolates). Two primers (H1 & H2) were used to study the genetic variation of E. coli and the electrophoresis band pattern was analyzed by the NTSYS.Results: The analysis of the difference in isolates using the RAPD technique showed a genetic similarity between 14% and 100%.Conclusion: The phylogenetic groups B2 and A were more frequent in fecal isolates. In addition, the number of isolates related to phylogenetic groups B1 and D was significantly lower than that of the other groups.
Highlights
Escherichia coli is one of the most prevalent bacterial species which cause gastrointestinal and digestive tract infections in humans and livestock
This study examined genotypic diversity of the E. coli isolates taken from fecal specimens in Zabol using random amplification of polymorphic DNA (RAPD) method and phylogenetic background
The phylogenetic distribution of the E. coli isolates was determined by the Triplex-polymerase chain reaction (PCR), as described by Clermont et al, using primers provided in Table 1.4 5 μL of the PCR product was loaded in 2% agarose gel, stained with ethidium bromide, and visualized using a Gel-Doc (LOURMAT, France) device (Figure 1)
Summary
Escherichia coli is one of the most prevalent bacterial species which cause gastrointestinal and digestive tract infections in humans and livestock. This study examined genotypic diversity of the E. coli isolates taken from fecal specimens in Zabol using random amplification of polymorphic DNA (RAPD) method and phylogenetic background. Materials and Methods: In this study, 100 isolates were collected from human samples and identified by the common biochemical tests. Escherichia coli is a type of bacteria which can colonize and survive in numerous environmental habitats and animal hosts. Phylogenetic studies conducted with the polymerase chain reaction (PCR), multilocus enzyme electrophoresis, and ribotyping methods are of paramount importance in evaluating the genetic evolution of the E. coli. Identifying the phylogenetic groups of E. coli strains by PCR method is facilitated through determining the presence of yjaA and chuA genes and the TSPE4.C fragment.
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