Abstract
2-Naphthalene sulfonate (2NS) is a sulfonated aromatic compound and a momentous intermediate involved in the synthesis of dyes and surfactants. Thus, the present experiment was undertaken to evaluate the variation in biochemical constituents in liver and kidney of fresh water fish, Channa punctatus, after 2NS intoxication. After determination of lethal dose (LD) two sublethal doses, i.e. 0.33mg/15 g body weight (one-half of LD50) and 0.16mg/15 g b.w. (one-fourth of LD50) were selected for analyzing oxidative stress, genotoxicity and bioaccumulative potential of 2NS. Highest significant increase in oxidative stress and DNA damage in the exposed groups as compared with control group (P≤0.05) was observed at 96h. However, decreased values of all the studied parameters after 30days indicate repair capacity of fish. In order to study the alterations observed in biomolecules including lipids, proteins and nucleic acids, histopathology along with spectroscopic analysis using attenuated total reflection-Fourier transform infrared was also performed for 96 h exposed group. In addition, protein secondary structure analysis was focused in this study, which reveals alterations in α-helix and β-sheet structure after 2NS intoxication. Furthermore, the bioaccumulative potential of 2NS was revealed using high-performance liquid chromatography showing 1.83 and 45.54μg/ml concentration of 2NS in liver and kidney homogenate, respectively. As the study revealed 2NS as the potential health hazard to aquatic organisms, it entails the augmentation and adoption of pertinent policies regarding the management of such toxic compounds.
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