Abstract
AbstractScreening of a population of Ocimum basilicum var. glabratum Benth., raised from seeds collected from France, West Africa and Kerala (India) was carried out for chemical composition of its essential oils. In all three collections, plants were found to be comprised of 3 chemotypes which were morphologically indistinguishable, but each chemotype was found to be capable of intercrossing freely with the other two.Selfing was carried out during 4 consecutive generations, after which three pure lines were established. Study of the selfed progeny of each collection exhibited an interesting segregation in S2 and S3 generations. Along with segregation in F2 and F3 generations of various hybrids the results can be explained by assuming a gene ‘M’ existing in 3 (or more) allelic forms: The allele M0 for biosynthesis of estragole (methyl chavicol) is dominant to allelcs M1, for eugenol and M2 for camphor. Also, allele M1 is dominant to allele M2. The assumption was further confirmed by testcrosses of F1 hybrids of each cross with their recessive parent.
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