Abstract
Mature sorghum herbage is known to contain several water-soluble secondary metabolites (allelochemicals). In this study, we investigated quantitative trait loci (QTLs) associated with allelochemical characteristics in sorghum using linkage mapping and linkage disequilibrium (LD)-based association mapping. A sorghum diversity research set (SDRS) of 107 accessions was used in LD mapping whereas, F2:3 lines derived from a cross between Japanese and African landraces were used in linkage mapping. The QTLs were further confirmed by positional (targeted) association mapping with Q+K model. The inhibitory effect of water-soluble extracts (WSE) was tested on germination and root length of lettuce seedlings in four concentrations (25%, 50%, 75% and 100%). A Significant range of variations was observed among genotypes in both types of mapping populations (P < 0.05). A total of 181 simple sequence repeats (SSRs) derived from antecedently reported map have been used for genotyping of SDRS. A genetic linkage map of 151 sorghum SSR markers was also developed on 134 F2 individuals. The total map length was 1359.3 cM, with an average distance of 8.2 cM between adjacent markers. LD mapping identified three QTLs for inhibition effect on germination and seven QTLs for root length of lettuce seedlings. Whereas, a total of six QTLs for inhibition of germination and ten QTLs for root length were detected in linkage mapping approach. The percent phenotypic variation explained by individual QTL ranged from 6.9% to 27.3% in SDRS and 9.9% to 35.6% in F2:3 lines. Regional association analysis identified four QTLs, three of them are common in other methods too. No QTL was identified in the region where major gene for sorgoleone (SOR1) has been cloned previously on chromosome 5.
Highlights
Every living organism interacts with others and form an ecological system on the earth
The allelopathic effect of each accession was calculated based on inhibition of germination and root length of lettuce seeds treated with water-soluble extracts (WSE) extracted from it at six-leaf stage
Correlation among traits studied in sorghum diversity research set (SDRS) and F2:3 lines shows germination of lettuce seeds treated with 25% WSE had non-significant relationship for all other traits (Table 1)
Summary
Every living organism interacts with others and form an ecological system on the earth. Such interactions between organisms at several levels have been long known and studied. QTL mapping for allelopathic characteristics in sorghum Html) postdoctoral fellowship for overseas researchers FY2010-2011 to TS. We are thankful to Qatar National Library (QNL) for paying all article processing charges to journal. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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