Genetic analysis of micro propagated plants of Tinospora cordifolia (Willd.) Miers by using RAPD–PCR markers

Abstract

Random Amplified Polymorphic DNA (RAPD) is the simplest and cost-effective tool used for the analysis of the genetic fidelity of in vitro propagated plants of Tinospora cordifolia. A high-quality genomic DNA free from any contaminants, was isolated from five genotypes by the modified CTAB method. Out of 30 random decamer primers, OPA-02, OPA-03, OPA-09, OPA-10, OPB-02, OPB- 04, OPB-09 and OPK-06 showed fingerprints. Out of total 27 scored bands, 26 produced were monomorphic bands (96.2%) and one was polymorphic band (3.8%). The highest number of bands (6) were produced by OPA -03 and OPK-06 and minimum number of bands (1) was produced by OPB -04. The amplicon size varied from 200 bp to 1500 bp. A coefficient similarity value ranging from 0.87–0.93 was obtained. Maximum similarity value 0.93 was exhibited by both the genotypes namely, SB1 and SB3. From the present study, it has been concluded that the RAPD analysis can be used to check the genetic fidelity in the micro propagated plants of Tinospora cordifolia.