Genetic analysis of low-density polyethylene degrading bacteria from plastic dump sites

P Jayashree Lakshmi

doi:10.17485/ijst/v13i48.2066

Abstract

Objective: The principle goal of this examination is to screen bacteria having the ability to debase Low density polyethylene. Bacteria were disengaged from different plastic dump locales. Methods: The secludes acquired were screened for their capacity to use polyethylene as sole carbon source in the mineral salt medium (MSM). Among the isolated strains, Pseudomonas putida and Pseudomonas fluorescens could able to utilize Low Density Polyethylene (LDPE) as carbon source. Further to the preliminary screening, gene specific primers for alkane monooxygenase was synthesized and gradient PCR was performed to determine the presence of alkane monooxygenase gene in the bacterial isolates. Results: Among the four bacteria selected, three bacteria such as Pseudomonas putida and Pseudomonas fluorescens could able to express ALKB gene. Hence, those two bacteria may produce the key enzyme alkane monoxygenase, which is a crucial enzyme for the biotransformation of many xenobiotic compounds including LDPE. Conclusion: From this investigation it is inferred that organisms local to soil can possibly degradeplastic with proper method of time. Keywords: Low density polyethylene; Biodegradation; Bacteria; alkane monoxygenase; invitro Biodegradation assay

Highlights

Plastics are engineered polymers comprising little natural monomer units joined into a long chain by covalent bonds shaped during a polymerization response
Presence of alkB gene in the selected strains were determined to rule out its efficacy to be explored as potential microbe to be employed for biodegradation of Low Density Polyethylene (LDPE)
Soil samples were collected from four different plastic dump sites in Chennai to isolate polyethylene (LDPH) degrading bacteria from soil

Summary

Introduction

Plastics are engineered polymers comprising little natural monomer units joined into a long chain by covalent bonds shaped during a polymerization response. Natural and inorganic crude materials, for example, carbon, silicon, hydrogen, nitrogen, oxygen and chloride are utilized for the production of plastics. Cremation, reusing and land filling are a portion of the customary techniques for taking care of plastic squanders. Be that as it may, these techniques are exorbitant and regularly make new ecological troubles. [6] Natural debasement is the wonder of organic change of natural mixes by living creatures especially microbes. Presence of alkB gene in the selected strains were determined to rule out its efficacy to be explored as potential microbe to be employed for biodegradation of LDPE. In that Psuedomonas spp. showed the presence of alkB gene

Materials and Methods

Results and Discussion

Conclusion