Abstract
The current study was aimed to detect the prevalence of Serratia fonticola strains in birds existed at birdmarkets and houses in different areas of Iraq. One hundred fifty Samples were collected from the wounds and tracheal swab in birds. Samples were cultured on MacConkey and blood agar. Identification of isolate was made according to colony morphology, Gram staining, and conventional biochemical interactions and analysis profile Vitek® 2 compact system. The culture results were revealed an overall one positive isolate with detection an antimicrobial susceptibility test by Vitek® 2 compact system and isolate showed that was susceptible to (Cefepime, Ceftazidime, Amikacin and Ciprofloxacin). In contrast, absolute resistant against (Aztreonam, Gentamicin and Trimethoprim/Sulfamethoxazole). Positive culture isolate was confirmed as Serratia fonticola by monoplex PCR assay, targeting 16S rRNA gene. Positive DNA product were sequenced, and recorded in the NCBI under the accession number (MN696514.1) for birds. The NCBI-BLAST Homology sequence identity reported a significant similarity between these local Serratia fonticola isolates and NCBI isolates; South Korean ( MK235160.1), Egyptian (MN865872.1), Russians (LT555292.1) Iranian (MK737094.1) isolate, at 99%. For our knowledge, this is the first molecular reports performed in Iraq for detection Serratia fonticola infections in birds in Iraq.
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