Abstract
Peroxidases, acting as oxidase upon appropriate substrates, generate carbonyl compounds in the electronically excited triplet state. These excited species can transfer energy as demonstrated by the appearance of the acceptor fluorescence or induced photochemistry concomitant with the disappearance of phosphorescence. Chlorophyll, an efficient emissive acceptor, either naturally present or artificially incorporated into organelles and cells, allows the in situ detection of biologically generated excited species. With neutrophils, the myeloperoxidase promoted acetone phosphorescence can readily be detected. In other cases, e.g. triplet benzaldehyde, it is possible to observe emission from lipid peroxidation initiated by the triplet carbonyl compound.
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