Abstract
Endospores are desirable phenotypes in Microbially-Induced Calcium Carbonate Precipitation (MICCP) for their durability in challenging environments. This study investigates the impact of various endosporulation methods on endospore morphology and their subsequent performance under harsh conditions. Results revealed that the Thermal Shock (TS) method produced larger endospores, possibly due to multi-layer encasement. Notably, endosporulation and germination ratios, besides MICCP effectiveness, achieved via TS-produced endospores surpass that of the Carbon Starvation method, both in vitro and within a cement-paste setting under freeze and thaw cycling. This outcome underscores the pivotal role of utilizing TS-produced endospores for concrete self-healing, particularly in cold areas.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
