Abstract
© 2015 O. M. Malanchuk et al. Aim. Generation of monoclonal antibodies specific to Coenzyme A. Methods. Hybridoma technique. KLH carrier protein conjugated with CoA was used for immunization. Screening of positive clones was performed with BSA conjugated to CoA. Results. Monoclonal antibody that specifically recognizes CoA and CoA derivatives, but not its precursors ATP and cysteine has been generated. Conclusion. In this study, we describe for the first time the production and characterization of monoclonal antibodies against CoA. The monoclonal antibody 1F10 was shown to recognize specifically CoA in Western blotting, ELISA and immunoprecipitation. These properties make this antiboby a particularly valuable reagent for elucidating CoA function in health and disease.
Highlights
Introduction vel ofCoA occur at several pathological conditions, such as diabetes, cancer and cardiac hypertrophy [4–Coenzyme A (CoA) is an essential cofactor in all liv- 12]
Coenzyme A was discovered by Lipmann in 1945 as a heat-stable cofactor required for many enzyme-catalysed acetylation reactions
CoA has a unique structure which allows it to function as a master acyl group carrier and carbonyl-activating group, resulting in a diverse range of metabolically active thioester derivatives, including acetyl-CoA, malonyl-CoA, 3-hydroxy-3-methylglutaryl-CoA etc (Fig. 1)
Summary
Coenzyme A (CoA) is an essential cofactor in all liv- 12]. Defective CoA biosynthesis is implicated in neuing organisms. CoA and its thioester derivatives (ace- rodegeneration with brain iron accumulation (NBIA). Tyl-CoA, malonyl-CoA, HMG-CoA etc) participate [13,14,15]. The exact role of CoA and its dein the diverse anabolic and catabolic pathways. Apart rivatives in the pathogenesis of the above disorders from participating in cellular metabolism as sub- is not well understood. The reliable and accurate asstrates and intermediates, CoA and its derivatives, says for measuring CoA species in biological samsuch as acetyl-CoA, can directly regulate the ac- ples are essential for better understanding of the tivity of proteins by allosteric mechanisms and gene roles of CoA and CoA derivatives under physiologiexpression by protein acetylation [1]
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