Abstract
There is a high demand for the development of in vitro models for human brain development and diseases due to the inaccessibility of human brain tissues. The human iPSC-derived brain organoids provide a promising in vitro model for studying human brain development and disorders. However, it is challenging to generate a large number of brain organoids with highconsistency for modeling human neurological diseases. Here, we describe a method for generating high-yield brain organoids with highconsistency by combining large-scale embryoid body (EB) generation and incorporating a quality controlscreening step duringdifferentiation. The method described in this chapter provides a robust way to generate brain organoids for studying human brain development and modeling neurological diseases.
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