General diversity of marbled eel (Anguilla marmorata) population in central of Vietnam, based on 16S rRNA sequences by barcode DNA

Abstract

The marbled eel has a distribution with great profitable, ecological and conservative values. Studies on the biology, population structure and phylogenetic of marbled eel are still incomplete. The research uses the large subunit ribosomal RNA (16S rRNA) of mitochondrial sequences to evaluate the genetic diversity populations of marbled eels living in the different ecosystems the regions in Central Vietnam. There are 7 polymorphic sites. 8 haplotypes, haplotype diversity (Hd) = 0.534 ± 0.079 and nucleotide diversity (π) = 99 x 10-3 were identified. The negative values are based on analysis of the neutrality tests, Strobeck’s S (S) values between individuals in populations = 0.00 to 1.000 and the genetic intraspecific distance between (Fst) individuals are in the population = 0.0000 – 0.0032 (mean = 0.0010). The individuals in the population signs of recent population expansion. A random pattern of genetic evolution with a high probability of occurrence of rare alleles as populations expand geographically has been found in the population. All marbled eels collected from Central Vietnam were genetically closely related. This result lays the foundation for the conservation strategy of the species germplasm resources and develops this species in Central Vietnam.