Abstract
DNA methylation in gene or gene body could influence gene transcription. Moreover, methylation in gene regions along with CpG island regions could modulate the transcription to undetectable gene expression levels. Therefore, it is necessary to investigate the methylation levels within the gene, gene body, CpG island regions, and their overlapped regions and then identify the gene-based differentially methylated regions (GeneDMRs). In this study, R package GeneDMRs aims to facilitate computing gene-based methylation rate using next-generation sequencing-based methylome data. The user-friendly GeneDMRs package is presented to analyze the methylation levels in each gene/promoter/exon/intron/CpG island/CpG island shore or each overlapped region (e.g., gene-CpG island/promoter-CpG island/exon-CpG island/intron-CpG island/gene-CpG island shore/promoter-CpG island shore/exon-CpG island shore/intron-CpG island shore). GeneDMRs can also interpret complex interplays between methylation levels and gene expression differences or similarities across physiological conditions or disease states. We used the public reduced representation bisulfite sequencing data of mouse (GSE62392) for evaluating software and revealing novel biologically significant results to supplement the previous research. In addition, the whole-genome bisulfite sequencing data of cattle (GSE106538) given the much larger size was used for further evaluation.
Highlights
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CpG/differentially methylated cytosines (DMCs) involved in this gene and Wij is the weight of reads of the involved CpG/DMC j of individual i
GeneDMRs is defined by the comparisons across different treatment groups following logistic regression model: ln
Summary
General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Published in: Proceedings of 27th Conference on Intelligent Systems for Molecular Biology and 18th European Conference on Computational Biology Citation (APA): Wang, X., & Kadarmideen, H.
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