Gene Over-Expression of Glycerol-3-phosphate Dehydrogenase in Some Marine Algae of Egypt.

Abstract

Studies focusing a survey or a comparison between freshwater and marine algae are relatively Algae exhibit a wide range of acclimation in response to induced salinity. However, studies focusing a survey or a comparison between freshwater and marine algae are relatively scarce. A comparative study between three fresh water algae (Spirogyra sp., Chara sp. and Cladophora sp.) and six marine algae among them brown: Sargassum dentifolium, Padina boryana, Dictyota dichotoma; red: Gelidium latifolium, Gracilaria dura and green: Enteromorpha intestinalis) has been performed to study how marine algae can cope with salinity of seawater. Algal fluorescence from the marine and fresh water groups was analyzed to determine whether differences in room-temperature fluorescence could be ascribed to differences between algal classes or specific for individuals. Results obtained showed that fluorescence signatures were relatively uniform for species within class but were different for different classes. Scanning of the SDS-gel showed the presence of certain characteristic protein banding pattern for each species. The amount of proline showed irregular differences according to species. Mannitol was detected only in the members of brown algae. Moreover, the mineral (sodium and potassium) and glycerol contents of the marine algae has noticeable greater values than those of fresh water members. The expression level of G3PDH mRNA was significantly upregulated in marine algal group as compared to that of the freshwater algae. GPDH had a significant impact on numerous metabolic processes in marine algae, including the biosynthesis of glycerol.