Abstract
In this study, two isolates A. lowffii, and A. baumannii were obtained clinically, and genomically identified by 16S rRNA assessment with the accession number (MH685113.1 and MH685112.1) for A. lowffii and A. baumannii respectively. The sensitivity profile of the isolates was variable, and A. baumannii was the most resistant strain towards a wide range of antibiotics, and it did not show any growth defect in the presence of β-lactam antibiotic, in comparison with A. lowffii. We identified that the blaOXA-23 gene was responsible for imipenem resistance in A. baumannii, whereas, blaOXA-51-like was moderately confer resistance towards A. lowffii which lack of blaOXA-23. This was determined when the isolates were subjected to qRT-PCR. We identified that the blaOXA-23 gene was increased about 1-fold in the presence of imipenem, whereas, blaOXA-51-like did not increased in comparison to the control. Bioinformatic analyses revealed that blaOXA-23 is located in the cytoplasm, and blaOXA-like 51 is located in the periplasm. Our hypothesis suggests that blaOXA-23 gene have a major contribution in the outbreak of multidrug resistance Acinetobacter species.
Highlights
Acinetobacter spp. is a non fastidious Gramnegative bacteria, belongs to gamma protobacteria, nonfermenting, stringency aerobic and non motile
We demonstrate that the expression of blaOXAlike 51 and blaOXA-23 is variable in response to imipenem addition in A. baumannii and in A. lowffii
Since the 16S rRNA gene was developed as a new standard for identifying bacteria, a wide range of bacteria has been determined by 5S, 16S and 23S rRNA gene sequences [20,56]
Summary
Acinetobacter spp. is a non fastidious Gramnegative bacteria, belongs to gamma protobacteria, nonfermenting, stringency aerobic and non motile. Acinetobacter species has been known for their ingenuin resistance to antibiotics, and for their ability to acquire genes encoding determinants, and other mechanisms that involved β-lactamases production and aminoglycoside-modifying enzymes [2]. Bacterial growth curve reactions were set up as detailed in was performed either under normal conditions, manufacturers’ guideline with a modification or with the presence of antibiotic. DNA Manipulation and Isolation, 16S qRT-PCR Analyses Acinetobacter cultures were grown in triplicate in MH broth under normal aerobic conditions rRNA Sequencing, PCR for 24 hr.
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