Gene Expression Analysis in Nasal Polyp Using Microarray

Abstract

Background and ObjectivesZZA nasal polyp is a chronic inflammatory disease that af fects 1~4% of the general adult population. It is characterized by edematous inflamed mu cosa prolapsing into the nose, leading to nasal obstruction, abnormal secretions, loss of smell, headache, and a reduced quality of life. We sought to screen for diseases related genes in nasal polyp by using DNA microarray and to validate the altered expression of selected genes. Subjects and MethodZZThe samples were obtained from nine patients with nasal polyps and two patients with pituitary adenoma. DNA microarrays containing 22,063 genes were used to compare individual gene profiles of nasal polyp samples and normal mucosal sam ples. A study of mRNA expression of selected genes was performed using RT-PCR. ResultsZZ126 genes were up-regulated at least 2-fold, and 161 genes were down-regulated at least 50% in nasal polyps compared with the normal sinus mucosa. The three most upregulated genes (STATH, TCN1, DMBT1) and one most down-regulated gene (SCGB1A1) were retested by means of RT-PCR. RT-PCR confirmed the differential expressions of all the genes in nasal polyp compared with normal sinus mucosa. ConclusionZZThis study could provide a clue for investigating possible pathophysiologic processes involved in nasal polyps. � Korean J Otorhinolaryngol-Head Neck Surg 2011;54:55-61