Gelatinisation Characteristics and Enzyme Susceptibility of Different Types of Barley Starch in the Temperature Range 48-72°C1

Abstract

The gelatinisation temperatures, pasting characteristics and enzymic susceptibilities in the temperature range 48-72°C of normal, high amylose, low amylose and zero amylose barley starches were determined. Normal starches had the lowest gelatinisation temperatures, but low and zero amylose starches had the lowest pasting temperatures. Normal starches were the most readily soluble in water at 48-60°C in the presence of a mixture of α-amylase, β-amylase and limit dextrinase and were most readily broken down to reducing sugars by these enzymes. High amylose starch was the most resistant to enzymic hydrolysis in the temperature range 48-72°C and, hence, produced the lowest level of reducing sugars.