Abstract
AbstractComparisons have been made between gel permeation chromatograms obtained at solvent flow rates of 1.0 and 4.5 ml/min. Using a 24 ft column series packed with Bio Glas the pressure drop was 200 lb/in2 which is within the operating range of all gel permeation chromatographs and is considerably less than when crosslinked polystyrene gels are used. The chromatograms from the Bio Glas packed columns exhibited skewing, similar to that reported previously for polystyrene gel packed columns. An analysis of these chromatograms indicated that the increased peak width caused by the faster flow rate was serious. Typically Mw/Mn values calculated at 4.5 ml/min were double their true values. Additionally skewing badly distorted the differential molecular weight distribution for molecular weights of 411 000 and higher. Previous studies in this area had not described quantitatively this adverse effect of increasing solvent flow rate in gel permeation chromatography.
Published Version
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