Abstract
To investigate the characteristics of Gd-EOB-DTPA MRI imaging tumor cell apoptosis and the correlation between them in orthotopic rats model of hepatocellular carcinoma after stereotactic radiotherapy. Forty healthy male Sprague-Dawley rats were randomly divided into model group (n=40) and control group (n=20). When the tumor diameter was ≥1.0cm, the rats in the experimental group underwent single stereotactic radiotherapy with a dose of 15Gy. 40 rats after radiotherapy were randomly divided into 4 groups, 10 in each group. The control group was randomly divided into 4 groups of 5 each, and the above group was added. MR rats were routinely scanned and DWI scans were performed on the 1st, 7th, 14th, and 21st day after radiotherapy. The region of interest (ROI) ADC value and signal intensity (SI) of the tumor and normal life after radiotherapy were measured, and the ratio of ADC to SI (tumor/liver) was calculated. After the end of the scan, the animals were sacrificed, and the liver cell tumor specimens were subjected to DNA nick end labeling (TUNEL) to determine the apoptosis of the tumor cells, and the apoptotic index (apoptotic cells/tumor cells) was calculated. Two independent sample t-tests were used to compare the apoptotic index and ADC ratio between the radiotherapy group and the control group. Comparison of apoptotic index and ADC ratio between the radiotherapy groups was analyzed by one-way analysis of variance; the correlation between the two was performed using the Pearson test. The ADC and SI ratios were statistically significant at 7 days, 14 days and 21 days after radiotherapy (P<0.01=, there was no significant difference at 1 day after radiotherapy (P>0.05). 1 day, 7 days, 14 days, The apoptotic index of the 21-day radiotherapy group was significantly different from that of the control group (P<0.05=. The ADC and SI ratios were positively correlated with the apoptotic index (AI) (P<0.05). The imaging characteristics of Gd-EOB-DTPA MRI can reflect the dynamic changes of apoptosis at different time points after stereotactic radiotherapy in rat liver cancer.
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