GC-MS profiling and phytochemical quantification in methanolic extract of Bidens tripartita

Abstract

Bidens tripartita (Asteraceae) is a weed like plant commonly known as Burmarigold. It is found in water logged and wet regions. The leaves of Bidens tripartita were harvested, dried and grounded. The powdered leaves were extracted with methanol, the extract was processed into a paste and stored in the refrigerator. Standard quantitative spectrophotometric methods were applied to quantify total phenol, flavonoid and alkaloid. Gas chromatography mass spectrometry (GC-MS) evaluation of methanolic leaf extract of Bidens tripartita was according to standard procedures. The phytochemical results showed total phenol 83.35±1.17 mgGAE/g, total flavonoid 10.12±0.05 mgQE/g, total alkaloid 4.5±0.73 %. The GC-MS also reveals many Medicinal compounds like Caryophyllene, Spathulenol, N-butyl-benzenesulfonamide, Methyl palmitoleate, 9,12-octadecadienoic acid methyl ester, Phytol, Gamma tocopherol, Vitamin E, Campesterol, Stigmasterol. Conclusively Bidenstripartita contains a lot of Medicinal phytochemicals that can be harnessed through extensive extraction for the development of a drug.