GC-MS analysis and Larvicidal activity of Plectranthus amboinicus and Sphagneticola calendulacea leaf extract in relation to larvicidal activity against the mosquito Aedes aegypti

Abstract

Many of the vector-borne diseases that harm humans and animals are transmitted by mosquitos. Malaria, filariasis, Japanese encephalitis, dengue fever, dengue haemorrhagic fever and yellow fever are all transmitted by mosquitos. Aedes aegypti and Anopheles stephensi are two mosquito species that are vectors. Yellow fever, dengue fever and chikungunya are all spread by Aedes aegypti, which is found across the tropical and subtropical zones. In India and other west Asian nations, A. stephensi is the predominant malaria vector. In both the Plectranthusamboinicus and Sphagneticolacalendulacea plant extracts, GC-MS analysis revealed 27 compounds. The mosquitocidal effectiveness of Plectranthusamboinicus and Sphagneticolacalendulacea ethyl acetate extracts against Aedes aegypti and Anopheles stephensi was investigated. Third and fourth instar larvae of Aedes aegypti and Aedes stephensi were killed after 24 and 48 hours of treatment in control by 50, 100, 120, 140, 150, 160, 180, 200, 250, 300 and 350 ppm concentrations respectively. The plant extracts were screened to identify the phytochemical bioactive compounds. A. aegypti was found to be most susceptible than the other species. LC50 and LC90 values of ethyl acetate extract were calculated against Aedes aegypti and Anopheles stephensi. The extracts of the plant showed potent larvicidal efficacy and can be considered for further investigation.