GC/GC-MS analysis and biological activities of Lantana Camara Linn.

Abstract

Medicinal plants have been a part of human history for thousands of years and are still used as healthcare throughout the world. The current research aims to explore the chemical constituents of the methanol soluble extract (LC-Me) and petroleum ether soluble fraction (LCM-PES) from the leaves of Lantana camara Linn by GC/ GC-MS. This chemical analysis revealed the existence of 16 and 23 phytoconstituents in LC-Me and LCM-PES respectively. The major constituents in LC-Me were found to beethyl 9,12,15-octadecatrienoate (31.9%), hexadecanoic acid, ethyl ester (12.6%), n-hexadecanoic acid (11.1%), linoleic acid ethyl ester (9.1%), squalene (8.7%), di-n-octyl phthalate (6.2%), 9,12-octadecadienoic acid (Z,Z)- (4.2%), (E)-9-octadecenoic acid ethyl ester (2.7%) andcyclopropanebutanoicacid,2-[[2-[[2-[(2-pentylcyclopropyl)methyl]cyclopropyl]methyl]cyclopropyl]methyl]-, methyl ester (2.3%). The chief bioactive compounds in petroleum ether soluble fraction were found to beandrost-8-en-3-ol, 4,4,14α-trimethyl-17-(2-bromo-1-methylethyl (57.9%), 14,17-nor-3,21-dioxo-β-amyrin, 17,18-didehydro-3-dehydroxy- (13.0%), barringtogenol B (2.5%), olean-12-ene-3,16,21,22,28-pentol, 21-(2-methyl-2-butenoate), [3β,16α,21β(Z),22α]-(1.7%),perhydrocyclopropa[e]azulene-4,5,6-triol, 1,1,4,6-tetramethyl (1.7%), ethyl iso-allocholate (1.6%) and 1,2-benzenedicarboxylic acid, diisooctyl ester (1.6%). Both the extract and its fraction have exhibited very significant antibacterial, antifungal,mosquito repellent and larvicidal propertiesoriginated by numerous bioactive metabolites. Twenty eight (20 Gram-positive and 8 Gram-negative) bacteria were tested against LC.Me and LCM-PESwith noteworthy zone of inhibition.The significant in vitro antifungal activity was observed against fifteen fungi in LC-Me and LCM-PES. Very robust initial repellency was observed for LC-Me and LCM-PES (94% and 80% respectively) against the dengue-carrying mosquito (Aedes aegypti) at 2% concentration. The extract and its fraction were also found to be an efficient larvicidal agent against fourth-stage larvae of Aedes aegypti. The effective larvicidal property was noted in methanol soluble extract as compared topetroleum ether soluble fraction and standard with LC50value of 20 and 400 ppm respectively.