Abstract
<p>This study aims to assess the GC-MS and FTIR profiles and secondary metabolites of <em>Ipomoea asarifolia</em> vis-a-vis its antibacterial action against bacteria, associated with wound infections. We conducted phytochemical screenings, GC-MS, and FTIR analyses on <em>I. asarifolia</em>.  The antibacterial effects of the extracts were tested against <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em>. Reports indicated the presence of saponins (57.9, 13.81, 46 mg/g), phenolics (18.4, 4.14, 21.05 mg/g), and alkaloids (0.49, 0.62, 1.12 mg/g) in the leaf, stem, and whole plant, respectively. <em>I. asarifolia</em> contained eighteen (18 of 20) essential amino acids, including glycine, alanine, serine, and proline. We also found phytosterol, fatty acids, phospholipids, and vitamins (B, C, D, E, and K) in the samples. We detected distinctive absorption bands in the leaf and stem samples, corresponding to the stretching of O-H bonds. The absorption band at 3419.95 cm<sup>−1</sup> was discovered in the leaf, while the absorption band at 3416 cm<sup>-1</sup> was observed in the stem. A prominent adsorption peak at 1637.83 cm<sup>−1</sup>, corresponding to the stretching of the alkene C=C bond in lignin, was detected in the leaf sample. The zone size inhibition for leaf and stem extracts ranges from 5 mm to 8 mm, depending on the extract concentration (0.625–5.0 mg/mL). <em>S. aureus</em> exhibited susceptibility to ciprofloxacin and norfloxacin but showed resistance to 15 other antibiotics. In contrast, <em>P. aeruginosa</em> displayed resistance to all tested antibiotics. The study provided confirmation and clarification of the traditional applications of <em>I. asarifolia</em>, a herbal plant that necessitates further investigation.</p>
Published Version
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