GC-MS Evaluation of Bioactive Constituents of Hydroalcoholic Leaves Extract of Pongamia pinnata with Antibacterial Potential of Prepared Mouthwash Formulations

Abstract

The leaves of Pongamia pinnata was extracted with 800 ml of 70% ethanol and 30% water (V/V) mixture for 48 hr. The hydroalcoholic extract of Pongamia pinnata leaves was subjected to preliminary phytochemical analysis. The presence of alkaloids, flavonoids, phenolic compounds, tannins and terpenoids was confirmed by preliminary phytochemical screening. The chemical composition of hydroalcoholic extract was determined by GC-MS analysis. The GC-MS investigation revealed the presence of sixteen different bioactive compounds in the Pongamia pinnata leaves extract. The least amount of constituents present in the hydroalcoholic extracts was dioctyl phthalate has the peak area 0.84 and maximum 38.17 peak area % was recorded for 3-Methoxy-1,2-propanediol. Three formulations (F-1), (F-2) and (F-3) of herbal mouthwash containing hydroalcoholic leaves extract of Pongamia pinnata were prepared. Then antibacterial activity of all three formulations of mouthwash were tested against Streptococcus mutans (oral pathogen). The results obtained were compared with standard mouthwash (Chlorhexidine). The findings revealed that prepared herbal mouthwash formulation 2 (F-2) showed significant zone of inhibition compared with standard Chlorhexidine.