GC-MS analysis, antioxidant, antibacterial and anticancer activities of methanol bark extract of Albizia lebbeck (L.)

Abstract

Albizia lebbeck is a deciduous tree with compound leaves, flat oblong fruits, round cream seeds are colored and wild growth. Albizia lebbeck is found throughout India, Bangladesh, tropical and subtropical Asia and Africa. For toothache and gum diseases, bark parts are essentially used. Extracted residues of the leaves and barks are protective against bronchial asthma and other allergic disorders. Barks and seeds are astringent and are used in the treatment of piles and diarrhoea. Current research studies were carried out for evaluating the antioxidant, antibacterial and anticancer potentiality of methanol bark extract of A. lebbeck and to identify the bioactive compounds by GC-MS analysis. Anticancer activity was carried out by MTT reduction assay method on MCF 7 cancer cell line. Antioxidant activities such as DPPH˙ radical, ABTS●+ radical cation, superoxide (O2 .- ) radical, phosphomolybdenum reduction and Fe3+ reducing power assays were carried out for bark extract. The maximum DPPH˙ radical scavenging activity was 90.97±0.36% at 120 µg/mL concentration and its IC50 was 35.89 µg/mL concentration. The maximum ABTS●+ radical cation scavenging activity was 95.65±0.30% at 30 µg/mL concentration and its IC50 was 13.79 µg/mL concentration. The maximum superoxide (O2 .- ) radical scavenging activity was 56.45±0.35% at 60 µg/mL concentration and its IC50 was 46.28 µg/mL concentration. The maximum phosphomolybdenum reduction showed 88.98±0.28% at 120 µg/mL concentration and Fe3+ reduction showed 80.55±0.34% at 120 µg/mL concentration. The RC50 of Mo6+ reduction and Fe3+ reduction were 31.39 µg/mL and 39.16 µg/mL concentration respectively. The maximum MCF 7 cell death was 79.36±0.23% at 250 µg/mL concentration and the IC50 was 47.12 µg/mL concentration.