GC-MS analysis and anti-malodor activity of Indonesian patchouli leaves essential oil [Pogostemon cablin (Blanco) Benth.] against Corynebacterium Sp isolate

Abstract

Most of the studies report that species of the Corynebacterium genus are generally causal bacteria of axillary odor, by converting odorless secretions compounds into odorous volatile molecules. The aim of this work was to provide information on the chemical compounds in the essential oil of Indonesian patchouli leaf (Pogostemon cablin (Blanco) Benth) that demonstrated the antibacterial activity against Corynebacterium sp. clinical isolate. The Corynebacterium sp. was isolated from basketball players' armpits and identified by observing the specific colonies characteristics on the blood agar. The chemical compounds were characterized using gas chromatography coupled to mass spectrometer (GC-MS). The antibacterial activity was performed according to the procedures of the Clinical and Laboratory Standards Institute. According to GC-MS result, the essential oil of Indonesian patchouli leaf presented 62 different compounds with the majorities as the following compounds: patchouli alcohol (21.00%), 1H-benzocycloheptene, 2,4a, 6,6,7,8-hexahydro-3,5,5,9-tetramethyl- (18.70%), caryophyllene (5.36%), seychellene (4.24%) and cyclohexene,1-methyl-4-(5-methyl-1-methylene-4-hexenyl) (3.01%). The integration of these compounds potentiates the antibacterial activity of the essential oil with lower concentration than 10 % v/v. Therefore, the essential oil of Indonesian patchouli leaf presented the significant antibacterial activity against Corynebacterium sp. as the causative agent of axillary odor.