Abstract
BackgroundWe investigated the effects of gastric Helicobacter pylori infection on the daytime and overnight human oral microbiota.MethodsTwenty four volunteers were recruited. Ten tested positive for H. pylori infection by the Carbon-14 Urea Breath Test, and the rest were negative. Two oral swabs were collected: one immediately after waking up in the morning and before brushing teeth, and another in the evening before teeth-brushing. DNA extract acquired from each swab was subjected to Illumina sequencing of 16S rRNA gene amplicons. The microbial abundance and composition were analysed in relation to H. pylori infection status.ResultsHelicobacter pylori-positive individuals had significant changes in the alpha and beta diversities in the daytime samples in comparison to those who were H. pylori negative. To identify which taxa could be significantly affected within the cohorts in the daytime, we employed the LEfSe method. When compared against UBT-negative samples, significantly higher abundances were detected in both Pseudomonas and Roseomonas, while Fusobacterium, Solobacterium, Haemophilus and Streptococcus were significantly decreased in the UBT-positive samples.DiscussionOur data demonstrated that H. pylori infection affects the human daytime oral microbiota. The hitherto undocumented changes of several bacterial genera due to H. pylori infection require more studies to examine their potential health effects on affected individuals.
Highlights
Helicobacter pylori is a highly adapted gastric pathogen that infects nearly half of the world’s population
The absence of Helicobacter sequence in our study could be attributed to our oral swab collection method that did not include any dental plaque material, or it may be that H. pylori was genuinely absent in the oral cavity of these Urea Breath Test (UBT)-positive individuals on the days covered by the samples
Our study reported the effects of H. pylori infection on the human oral microbiome, during the day
Summary
Helicobacter pylori is a highly adapted gastric pathogen that infects nearly half of the world’s population. It is an important etiological factor for the development of peptic and duodenal ulcerations, chronic gastritis and gastric atrophy. Gastric Helicobacter pylori infection perturbs human oral microbiota. We investigated the effects of gastric Helicobacter pylori infection on the daytime and overnight human oral microbiota. Helicobacter pylori-positive individuals had significant changes in the alpha and beta diversities in the daytime samples in comparison to those who were H. pylori negative. To identify which taxa could be significantly affected within the cohorts in the daytime, we employed the LEfSe method. Our data demonstrated that H. pylori infection affects the human daytime oral microbiota. The hitherto undocumented changes of several bacterial genera due to H. pylori infection require more studies to examine their potential health effects on affected individuals
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