Gaschromatographische untersuchungen der fettsäuren der neutralfette, phospholipide und cholesterinester reifer menschlicher placentazotten in vitro

Abstract

Zusammenfassung In mature human placental villi, the fatty acids of six lipid fractions were investigated by gas chromatography. In vitro incubation (2 to 180 min) shows a significantly changed composition of the following fatty acids (moles/100 moles fatty acids): 1. 1. In the fraction of free fatty acids C14: 0 and C18: 1 diminish between the 2nd and 30th min of incubation time; C14: 0 increases between the 30th and 120th min. Polyunsaturated fatty acids (C16: 1, C18: 2, C18: 3, C20: 4) are found. 2. 2. In the fraction of monoglyceridcs the fatty acids C16: 1, C18: 0 and C18: 1 increase between the 15th and 180th min of incubation. Polyunsaturated fatty acids are not detectable. 3. 3. In the fractions of diglycerides and triglycerides saturated (C16: 0, C18: 0, C22: 0) and unsaturated (C16: 1, C16: 2, C20: 4) fatty acids increase during the whole incubation time. 4. 4. In the fraction of phospholipids the saturated fatty acids decrease, but the polyunsaturated fatty acids (C16: 1, C20: 4) increase. 5. 5. In the fraction of cholesterol esters saturated fatty acids (C14: 0) decrease while the unsaturated fatty acid (C16: 1, C18: 1 C20: 4) increase. These alterations of the fatty acid composition of the six lipid fractions are discussed in view of the metabolism of these fatty acids, as during the in vitro incubation also the incorporation of [I-14C]acetate in these six lipid fractions changes significantly.