Abstract
A gas-liquid chromatographic (GLC) method has been developed for the analysis of samples containing nanogram to picogram amounts of three different types of monosaccharides, viz., aldoses, ketoses and deoxy sugars. The minimum detectable amounts were at the sub-picogram level when highly electron-capturing derivatives such as trifluoroacetyl (TFA) esters of sugars were used. The gas chromatograph was modified to utilize the powerful combination of a glass capillary column and an electron-capture detector. For nine monosaccharides all of the anomers were resolved on a glass capillary column with particularly good deactivation properties. The losses of the TFA derivatives on the column were determined approximately at different carrier gas flow-rates. Quantitative determination was facilitated by taking all anomeric forms into account. The sensitivity and resolution of the method permit extremely small amounts of sugars in a complex mixture to be studied. This has been demonstrated on a sea-water sample.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
