Abstract
A method for measuring the antitumor agent cyclophosphamide was developed and applied to the determination of the drug in biological specimens. After extraction with ether, cyclophosphamide and the internal standard isophosphamide are converted to their trifluoroacetyl derivatives and assayed by GLC, using either an electron-capture or a flame-ionization detector. The minimum detectable amount is 25 pg/injection using the electron-capture detector. Linearity was found up to microgram amounts of substance, without any interference of endogenous substrates. Values from serum, urine, and liver in mice treated with a single dose of cyclophosphamide (85 mg/kg) are also reported.
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