Abstract
The combination of immobilized alcohol oxidase (AOD) with a gas-diffusion membrane in a flow-injection system greatly enhanced the specificity of ethanol determination. This gas-diffusion flow-injection system with amperometric detection of hydrogen peroxide had a linear range of analysis from 0.0006 up to 60% (v/v) ethanol. Silicone-modified polypropylene membranes of different thickness were used. Undiluted samples of beer, wine, spirits and medicine could be investigated with excellent correlation with standard methods ( r > 0.997). The frequency of analysis was 120–180 samples h −1 and the operational half-life of the immobilized enzyme was 8000 injections within 44 h.
Published Version
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