Gas chromatography-mass spectroscopic (GC-MS) analysis of n-Hexane extract of &lt;i&gt;Lentinus tuber-regium&lt;/i&gt; (Fr) Fr (Polyporaceae) syn &lt;i&gt;Pleurotus tuber regium&lt;/i&gt; Fr sclerotia

Oe Afieroho,Kc Ugoeze

doi:10.4314/tjpr.v13i11.20

Abstract

Purpose: To identify the chemical constituents of the n-hexane extract of the sclerotia of Lentinus tuber-regium (synonym Pleurotus tuber regium) using gas chromatography-mass spectroscopic (GCMS) techniques. Methods: The n-hexane extract of the sclerotia of Lentinus tuber regium was obtained by exhaustive Soxhlet extraction and analysed using gas chromatography-mass spectroscopic (MS) techniques. The structures of the identified constituents were confirmed on the basis of their fragmentation pattern in comparison with that obtained from the National Institute of Standards and Technology (NIST) reference library. Results: Seven fatty acids derivatives: heptadecenal, n-hexadecanoic acid, 1-eicosene, linoleic acid, oleic acid, linoleic acid ethyl ester, and octadecanoic acid, and five steroidal triterpenoids: cholesterol, αergostenol, anthraergostatetraenol, stigmasterol, and alpha-ergosta-4,6,8(14),22-tetraen-3-one. The major constituents characterised are α-ergosta-4,6,8(14), 22-tetraen-3-one (8.56 %) > Anthraergostatetra-enol (7.19 %), > n-hexadecanoic acid (6.29 %) > linoleic acid (3.69 %). Conclusion: This study shows that L tuber-regium is a veritable source of mono- and poly-unsaturated fatty acids, and ergosterol/provitamin D derivatives which may explain, in part, some of its reported nutraceutical benefits.

Highlights

The result of the characterised chemical composition expressed as percent peak area response in Table 1 showed seven fatty acid derivatives (15.94 %) and five steroids (20.82 %) corresponding to 36.76 % of the total n-hexane extract
Of the total characterised fatty acids compositions, 44.42 % was due to unsaturated fatty acid constituents equivalent to 7.08 % of n-hexane extract
Oleic acid and linoleic acid derivatives were the major unsaturated fatty acids accounting for 6.14 % of total n-hexane extract, 86.72 % of the total characterised unsaturated fatty acid composition, and 38.52 % of the total characterised fatty acids

Summary

Introduction

(Pleurotaceae) commonly called the king tuber mushroom is an edible gilled fungus of the Agaricomycetes class. It grows wild in both tropical and subtropical regions of the world. It is a common mushroom in the southern part of Nigeria growing on dry wood, where it produces the large spherical to ovoid sclerotia which sometimes measure up to 30 cm in diameter [1,2]. The sclerotium is dark brown on the outside and white on the inside. It is called ‘katala’ in Hausa, ‘ike usu’ or ‘ero usu’ in Ibo, ‘awu’ in Igala and ‘umoho’usu’ in Igede (Nigeria)

Objectives

Results

Discussion

Conclusion