Abstract
The present investigation was carried out to characterize the bioactive compounds present in extract of Holothurian atra and Holothurian edulis using Gas Chromatography-Mass Spectrum (GC-MS) and antioxidan activity. The results of the GC-MS analysis provide different peaks determining the presence of 4 phytochemical compounds with different therapeutic activities. The measurement of antioxidant activity carried out using 1,1difenil-2-pikrihidazil (DPPH) method. The major, 9-Octadecenoic acid (Z)-, methyl ester (47.29), hexadecanoic acid, methyl ester (28.41), octadecanoic acid, methyl ester (4.47), and [1,1′-bibicyclo[2.2.2]Octane]-4-carboxylic acid (92.11) and minor compounds were also present. Antioxidant activity of the ethanolic extract of Holothurian edulis have low antioxidant activity with the value IC50 41.6828 mg/mL and ethanolic extracts of Holothurian atra have strong antioxidant activity with value IC50 33.514 mg/mL. From the results, it could be concluded that Holothurian atra and Holothurian edulis contains various bioactive compounds have various biological activities and antioxidant activity.
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