Abstract
A method for the determination of metoprolol at concentrations down to 10 ng/ml in human plasma is described. After addition of oxprenolol as internal standard, both compounds are extracted into diethyl ether—dichloromethane (4:1, v/v) at basic pH, transferred into an acidic aqueous solution and back-extracted at basic pH into diethyl ether—dichloromethane. They are then derivatized with heptafluorobutyric anhydride. The derivatives are quantitatively determined by gas chromatography using a 63Ni electron-capture detector. The linearity was demonstrated, and the technique was formally validated in the concentration range 10–500 ng/ml. The technique was applied in a study of the bioavailability of metoprolol after oral administration to man; mean plasma concentrations are reported.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
