Abstract
Previous researches focused on the chemical structure of Ganoderma atrum polysaccharide (PSG-1), and found it has antitumor and immunoregulatory activities. However, the mechanisms behind these biological activities remain unclear. The purpose of this study was to investigate the possible signaling pathways involved in the PSG-1 activated macrophage of S180 tumor-bearing mice. In vitro, PSG-1 probably stimulated macrophages resulting in an increase in phosphorylation of NF-κB, Akt and MAPK family proteins, which are indicative of activations of NF-κB pathway activation. Furthermore, the levels of TNF-α protein and TNF-α mRNA expression were significantly suppressed when macrophages were pretreated with various inhibitors, including NF-κB inhibitors, IκB inhibitors, MAPK inhibitors and PI3K/Akt inhibitors. These findings indicated the possible involvement of NF-κB signaling pathway in PSG-1-induced TNF-α secretion and mRNA expression, and provided new insights into the therapeutic potential of modulating NF-κB by PSG-1.
Published Version
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