Gangliosides in the blood plasma: levels of ganglio-series gangliosides in the plasma after administration of brain gangliosides

Abstract

The temporal change in the levels of the gangliotetraose-series gangliosides i.e., G M1a, G D1a, G D1b, G T1b, in the blood plasma after intramuscular administration of bovine brain gangliosides (5 mg/kg) to beagle dogs (11.3–12.2 kg) was determined with high sensitivity by a recently developed thin-layer chromatography/ enzyme-imnuinostaining method (Hirabayashi, Y., Koketsu, K., Higashi, H., Suzuki, Y., Matsumoto, M., Sugimoto, M. and Ogawa, T. (1986) Biochim. Biophys. Acta 876, 178–182). The amounts of G M1a, G D1a, G D1b, G T1b and their combined total in the plasma of beagle dogs before administration of gangliosides were 21 ± 1, 36 ± 7, 15 ± 2, 16 ± 2 and 88 ± 6 pmol/ml of blood plasma, respectively. Trapezoidal calculation showed that the times of the maximum levels of G M1a, G D1a, G D1b, G T1b and the total of the their levels in the plasma were 8.0 ± 1.2, 8.7 ± 0.7, 6.3 ± 2.0, 17.0 ± 7.0 and 8.7 ± 0.7 h after the administration of gangliosides, and their maximum concentrations were 517 ± 37, 654 ± 53, 160 ± 5, 184 ± 20 and 1383 ± 74 pmol/ml, respectively. The maximum level of each ganglioside decreased gradually, reaching the normal level after 10 days. The half-maximum level of each ganglioside occurred 2–3 days after the administration. Asialo G M1 (G A1) was not detected plasma at any of the test times.