Abstract
The mitochondrial, pinched-off nerve endings, and synaptic vesicle fractions prepared from rat brain have been shown to take-up and bind radioactive acetylcholine in vitro. This in vitro binding of acetylcholine by brain particles appears to be a passive proces;. A linear response exists between the amount of acetylcholine bound and the concentration of acetylcholine in the suspending sucrose solution. The slope of the response indicates 6μ moles of acetylcholine bound per g of nerve endings in 30 min in 0.1 M acetylcholine solution. The radioactive acetylcholine bound to the particles may be released by acid, heat, osmotic shock, freezing and thawing, exposure to lipid solvents, and incubation with neuraminidase. Using particles labelled with radioactive acetylcholine, it has been possible to show that gangliosides and acetylcholine are contained in the same subfraction obtained by sedimentation of the nerve endings fraction in a continuous sucrose gradient. A possible role for gangliosides in the transport of acetylcholine at the synapse is outlined.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
