Gamma (60 Hz) auditory stimulation improves intrusions but not recall and working memory in healthy adults

Abstract

Gamma-band (> 30 Hz) brain oscillations (γ) play a crucial role in memory and long-term potentiation, and their disruptions have been consistently documented in patients with Alzheimer's Disease (AD). Gamma-band oscillation entrainment through 60 Hz transcranial alternating stimulation (tACS) and 40 Hz tACS/sensory stimulation has been shown to enhance memory performance in healthy adults and patients with AD, respectively. However, the impact of gamma auditory stimulation on healthy adults' memory remains uncertain. In this balanced crossover study, 36 healthy subjects (27 Females) underwent three auditory stimulation conditions: no auditory stimulation (NO_AS), 40 Hz, and 60 Hz. Long-term verbal memory (LTM) and verbal working memory (WM) were assessed using, respectively, the Ray Auditory Verbal Test (RAVLT) and Digit Span Backward test (DS-B). We hypothesized that 60 Hz would improve LTM (as compared to NO_AS), but not WM; no specific effects were hypothesized for 40 Hz. We found that gamma-band auditory stimulation (40 Hz and 60 Hz) did not significantly affect RAVLT recall or WM. However, 60 Hz stimulation reduced RAVLT immediate recall intrusion; this outcome negatively correlated with DS-B performance, suggesting a positive impact of 60 Hz on executive functions. In summary, gamma-band auditory stimulation did not enhance memory in healthy adults, but 60 Hz stimulation potentially benefits executive functions. Further investigation is needed to understand gamma oscillation's role in cognitive processes for both healthy and clinical populations.