GalCer highly express in the lung and hepatocellular carcinomas

Abstract

Introduction Recent methods of genome analysis have advanced to the use of proteomics for small molecule detection. In addition, mass spectrometry (MS) is also used. We investigated the presence of small molecules expressed in pulmonary and hepatic neoplasms. We identified galactosylceramide (GalCer) was markedly expressed in tumor cells. Materials and methods Liquid chromatography MS (LC-MS) together with imaging MS was used to investigate the expression of small low molecules in tumor tissues obtained from lung and hepatic cancer patients. The localization and mRNA expression of GalCer sulfotransferase (GalCer-s), which synthesizes GalCer in tumor tissues, was analyzed. Results Previously, various small molecules were shown to be highly expressed in lung and hepatic cancer by imaging and LC-MS. Here, we observed that GalCer-s was highly expressed in neoplastic cells contained in tumor tissues obtained from lung and hepatic cancer patients. Conclusion It was previously reported that GalCer is involved in tumor immunity. Here, we demonstrated neoplastic cells highly expressed GalCer. The mechanism and role of GalCer expression in tumors must be considered in the future.