Abstract
Our objective was to develop and evaluate Ga-68- and Cu-64-labeled albumin conjugates for PET imaging of sentinel lymph nodes. Four different albumin conjugates were prepared starting from NOTA-HSA. The lymph node uptake of Ga-68- and Cu-64-labeled albumin conjugates was investigated after subcutaneous injection into the foot pad of Wistar rats. A pig model was utilized for further biological evaluation of the lymph node uptake. For all the four conjugates, radiolabeling with Ga-68 and Cu-64 resulted in >95% radiochemical yield. Denatured and mannosylated Ga-68 NOTA-HSA revealed the highest popliteal lymph node uptake in rats (2.78%±0.38% ID and 6.13%±1.13% ID 10 min and 60 min p.i., resp.). The popliteal lymph node reached its maximum activity after approximately 120 min and remained constant for denatured and mannosylated Cu-64 NOTA-HSA at least up to 240 min p.i. In a pig model, 2% of the injected dose of this compound was found in the sentinel lymph node 60 min after subcutaneous injection. In conclusion, PET imaging of sentinel lymph nodes with Ga-68- and Cu-64-labeled denatured NOTA-Man-HSA could be successfully demonstrated and deserves further investigations.
Highlights
2% of the injected dose of this compound was found in the sentinel lymph node 60 min after subcutaneous injection
Sentinel lymph node (SLN) diagnosis is an important field in nuclear medicine, and its benefit has been proven in many cancer types, mainly breast cancer and melanoma
We investigated the effect of human serum albumin (HSA) denaturation in combination with mannosylation and compared the biological performance of this agent with that of the nondenatured HSA as well as probes without mannosyl units
Summary
Sentinel lymph node (SLN) diagnosis is an important field in nuclear medicine, and its benefit has been proven in many cancer types, mainly breast cancer and melanoma. The sentinel lymph node is the first lymph node in the drainage basin of a primary tumor. SLNs are imaged by means of planar lymphoscintigraphy. The advantage of SPECT/CT in cases with difficult anatomical location of sentinel nodes was reported as an alternative imaging modality [1, 2]. In the second step of the procedure, SLNs are intraoperatively detected by a hand-held gamma camera, excised and pathologically examined. If the sentinel node is free of metastatic cells, the likelihood of general nodal metastasis is very low
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