Abstract
When a suspension of zoospores of Olpidium brassicae (Wor.) Dang. and tobacco necrosis virus (TNV) was added to seedling roots of mung bean ( Phaseolus mungo L.) and the seedlings were placed in a moist chamber 30 minutes later, infection of the roots by Olpidium and by TNV resulted. When the seedlings were placed in running tap water instead of in the moist chamber, TNV infection was increased. Infection was heavy in seedlings placed in TNV, exposed to prolonged washing, and inoculated with zoospores; apparently the zoospores transmitted TNV retained by the roots through the washing. Although zoospores do not complete root penetration until 2–3 hours after encystment, addition of TNV to roots 1–2 hours after zoospore encystment on the roots resulted in little or no TNV infection. When appropriate dilutions of TNV and antiserum were mixed and added to zoospores released into water, no virus transmission to roots resulted from the mixture, but transmission resulted when zoospores were released from roots into the virus suspension and the antiserum was added later. Zoospores released from TNV-infected roots into antiserum caused one-fifth to one-half as much TNV infection as similar zoospores released into normal serum. The addition of TNV antiserum to roots bearing zoospores that had encysted on the roots in the presence of TNV did not prevent TNV infection, although the antiserum inactivated the virus in suspension. Thus, the evidence indicates that the Olpidium zoospore acquires and harbors TNV in such a manner that antiserum cannot inactivate it. Therefore it is probably not merely a surface carriage. Unsuccessful attempts were made to transmit tobacco ringspot virus and tobacco rattle virus to Nicotiana tabacum L. ‘Xanthi’, tobacco mosaic virus and tomato bushy stunt virus to Lycopersicon esculentum Mill. ‘Bonny Best’, southern bean mosaic virus to Phaseolus vulgaris L. ‘Bountiful,’ and peach yellow bud mosaic virus to Cucumis sativus L. ‘National Pickling’, by adding combined suspensions of zoospores and the viruses to the roots.
Published Version
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