Further pharmacological study on the cholinergic and glutaminergic properties of the radula muscles of a mollusc, Rapana thomasiana

Abstract

Abstract 1. In the radula protractor of the prosobranch mollusc Rapana thomasiana , both twitch contractions and acetylcholine contractions were markedly depressed or blocked by propantheline (10 −5 M) and strychnine (10 −5 M), but in the radula retractor, only acetylcholine contraction was markedly affected by the antagonists, 2. Glutamate contractions of both of the muscles were little or slightly affected by the drugs. 3. Twitch contraction of the protractor was slowly depressed when the muscle was immersed in concanavalin A (0.3 mg/ml), while that of the retractor was first potentiated and then slowly depressed in it. 4. In both of the muscles, glutamate contractions were markedly enhanced by the lectin, but acetylcholine contractions were not affected. 5. These results support the notion that the principal excitatory neurotransmitter in the protractor is acetylcholine, whereas that in the retractor is glutamate.