Abstract
by us in comparative tests with antigens of different dilutions. The particular features of our test as finally recommended and the conditions under which it was conducted were that: (1) a dose of 0.02 ml of a 1:100,000 dilution of Dirofilaria immitis antigen was employed; (2) a corresponding concentration of dog serum was used as a control; (3) early and doubtful cases of filariasis, and individuals who had been in endemic areas, constituted the test group; and (4) a large number of persons, most of whom were allergic to various substances, but none of whom had ever lived in an endemic area, constituted the control group. It was concluded that a dilution of 1:100,000 of D. immitis in saline, in a dose of 0.02 ml, was a suitable antigen for the diagnostic test for filariasis.
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