Abstract
Defective, interfering particles of pseudorabies virus isolated after repeated (49) passages in rabbit kidney cells have been further characterized. The defective particles sediment at a slower rate in sucrose gradients than do standard virions. This difference in sedimentation rates is not due to a difference in the molecular weights of the DNA present in defective and standard virions; both types of particles contain DNA with a molecular weight of approximately 90 × 10 6. The content of DNA relative to protein is also the same for both types of particles (10–11 μg of DNA/100 μg of protein). Populations of purified defective and standard virions are homogeneous with respect to their content of DNA and protein. The defective particles become more highly labeled with glucosamine than do standard particles and the degree of glycosylation of some of the glycoproteins of defective particles differs from that of standard particles. The changed sedimentation behavior of defective virion populations may reside in the greater degree of glycosylation of some of their glycoproteins. It has proved impossible to separate by sedimentation the infectious virus present in the population of defective virions from the defective virions. The altered sedimentation behavior seems to be acquired by both infectious and defective particles produced by cells coinfected with defective and infectious virus.
Published Version
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