Abstract
It has been found that yeasts and yeast-like microorganisms were the stable constituents of denitrification unit biocenoses fed with mineral medium with methanol, glycerol, acetic or lactic acids. At an optimal C: N ratio, the number and composition of mycoflora depended on the type and structure of carbon compounds serving as hydrogen donors and C-sources as well as on volumetric nitrate loading rate. At nitrate loading rates below the “washing-out” points, the total number of these microorganisms was constant, about 10 times higher in biocenoses fed with organic acids rather than alcohols. Typical species for biocenosis fed with methanol, acetic acid and lactic acid were C. boidinii, C. famata, and C. famata and Hansenula californica, respectively. It is concluded that unit mycoflora utilize C-sources for denitrification, can assimilate nitrates and fix N 2 produced during nitrate dissimilation and stimulate the growth of denitrifying bacteria. The influence of yeasts growth in biocenoses on the course of the denitrification process is also discussed.
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