Fungal mediated silver nanoparticle synthesis using robust experimental design and its application in cotton fabric

Sulbha Girish Velhal,R. V. Latpate,S. D. Kulkarni

doi:10.1007/s40089-016-0192-9

Abstract

Among the different methods employed for the synthesis of nanoparticles, the biological method is most favorable and quite well established. In microorganisms, use of fungi in the biosynthesis of silver nanoparticles has a greater advantage over other microbial mediators. In this study, intracellular synthesis of silver nanoparticles from Aspergillus terrerus (Thom) MTCC632 was carried out. We observed that synthesis of silver nanoparticles depended on factors such as temperature, amount of biomass and concentration of silver ions in the reaction mixture. Hence, optimization of biosynthesis using these parameters was carried out using statistical tool ‘robust experimental design’. Size and morphology of synthesized nanoparticles were determined using X-ray diffraction technique, field emission scanning electron microscopy, energy dispersion spectroscopy, and transmission electron microscopy. Nano-embedded cotton fabric was further prepared and studied for its antibacterial properties.

Highlights

Silver is a naturally occurring precious metal, most often in the form of a mineral ore in association with other elements
Our X-ray diffraction study showed that silver nanoparticles of size ranging from maximum *13.80 to 2.0 nm were produced during synthesis
The Ultra violet–visible (UV–Vis) spectra indicated that the surface plasmon resonance of silver nanoparticles shifted to 290 nm due to cell-bound nature of nanoparticles

Summary

Introduction

Silver is a naturally occurring precious metal, most often in the form of a mineral ore in association with other elements. Silver is well-known for its strong antibacterial activity which leads to the development of various nanosilver products. Silver nanoparticles have been used in the medical field; wound dressing, surgical instruments, etc. [10, 11] have been in use Due to these extensive applications of silver nanoparticles, there is an increase in demand of their large scale synthesis with specific sizes and shapes. It is well-known that the properties of silver nanoparticles are greatly influenced by its size, shape and crystal structure. Silver nanoparticles synthesis with defined size and shape are at the leading edge of nanoscience and nanotechnology

Methods

Results

Conclusion