Abstract
The biosynthesis of silver nanoparticles and their notable in vitro antibacterial activities against E. coli, a Gram-negative bacterial strain are reported here. Spherical shaped silver nanoparticles with a diameter of 20 nm are prepared by a fungus Penicillium expansum. The formation process of the silver nanoparticles is investigated by UV-visible (UV-vis) spectroscopy, X-ray diffraction (XRD) measurements and transmission electron microscopy (TEM). Bacteriological tests were performed in Luria–Bertani (LB) medium on solid agar plates and supplemented with 3.10 μg/ml concentration of nanosized silver particles. These particles were shown to be an effective bactericide. Scanning electron microscopy (SEM) was used to study the biocidal action of this nanoscale material. The results confirmed that the treated E. coli cells were damaged, showing deform morphology exhibits a significant increase in permeability, resulting in death of the cell.
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