Biosynthesis and characterization of silver nanoparticles (AgNPs) using marine bacteria against certain human pathogens

J Nasrin Begam

doi:10.7439/ijasr.v2i7.3514

Abstract

The present work investigates the synthesis of silver nanoparticles (AgNPs) by biological method using marine bacteria. The minimum inhibitory concentration (MIC) test was performed to find the inhibitory concentration of AgNO3 against marine bacterial isolate. After MIC study, the biogenic AgNPs was prepared through marine bacterial isolate and characterized by using UV-visible spectroscopy, Scanning Electron Microscopy (SEM), X Ray Diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). From the UV-visible spectroscopy, the absorption peak was found at 420 nm. In SEM image, it is confirmed that the sample contains spherical shaped silver nanoparticles and most of the particles were below 100 nm in size. In XRD analysis, it was confirmed that the silver nanoparticles are crystalline in nature, which was confirmed by the FTIR peak at 518 cm-1 corresponding to the Ag vibration present in crystalline structure. The antimicrobial activity of silver nanoparticles was determined by disk diffusion method, and found that silver nanoparticles have significant antibacterial activity against most of the pathogens.

Highlights

IntroductionSilver nanoparticles (AgNPs) are one of the promising products in the nanotechnology industry
This study aims to synthesis of silver nanoparticles through biological method by using of marine bacterial isolate and it was characterized by several methods
Silver nanoparticle was successfully synthesizing by using of marine bacteria through biological method

Summary

Introduction

Silver nanoparticles (AgNPs) are one of the promising products in the nanotechnology industry. Silver nanoparticles can be synthesized by several physical, chemical and biological methods, in which, one of such promising process is green synthesis. An increasingly application is the use of silver nanoparticles for antimicrobial coatings, and many textiles, wound dressing, and biomedical devices contain silver nanoparticles that continuously release a low level of silver ions to provide protection against bacteria [1]. Some known examples of bacteria synthesizing inorganic materials include magnetotactic bacteria (synthesizing magnetic nanoparticles) and S layer bacteria which produce gypsum and calcium carbonate layers [2]. This study aims to synthesis of silver nanoparticles through biological method by using of marine bacterial isolate and it was characterized by several methods. The pharmacological applications such as antibacterial activity of AgNPs were studied against certain bacterial pathogens

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Conclusion